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KMID : 0605720010070020251
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Journal of the Korean Society of Biological Therapies in Psychiatry
2001 Volume.7 No. 2 p.251 ~ p.260
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The Effect of Antipsychotic Drugs on Cyelooxygenase-2 Gene Expression and Prostaglandin E₂ Level
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김종우/Kim, Jong-Woo
조성욱/범재명/윤도준/장환일/송지영/Jo, Seong Wook/Beom, Jae-Myoung/Yoon, Doh joon/Chang, Hwan-II/Song, Ji-Young
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Abstract
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Objectives : Cyclooxygenase(COX) is the central enzyme in the biosynthetic pathway to prostaglanciins from arachidonic acids. The action mechanism of nonsteroidal antiinflammatory drugs is through inhibition of COX activity and prostanoid biosynthesis including prostaglandin E2(PGE2). The antipsychotic drugs are known having an analgesic effects, however its mechanisms of action to pain control was not still clearly proved. In the present study, the effects of antipsychotic drugs(chlorpromazine and clozapine) on the production of PGE2 and the expression of COX-2 mRNA and protein in P815 cell line were studied.
Methods : For that purpose, the method: of RTPCR, western blot and prostagalndin E2 enzymeimmunoassay were applied.
Results :The results showed that in P81,`i cells treated with chlorpromazine, the level of COX2 mRNA was significantly decreased compared to the control cells. And total cellular PGE2 levels were significantly decreased after treatment with chlorpromazine and clozapine.
Conclusion :These results suggest that antipsychotic drugs have revealed their analgesic effect through the inhibition of COX2 activity and PGE2 production. And also, these results partly support the excess theory of prostaglandins in the pathogenesis of schizophrenia.
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